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oc43 strain vr 1558  (ATCC)


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    Structured Review

    ATCC oc43 strain vr 1558
    Oc43 Strain Vr 1558, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 460 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oc43+strain+vr+1558/Betacoronavirus+1/pm41857372-252-6-9
    Average 98 stars, based on 460 article reviews
    oc43 strain vr 1558 - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Binding Assay:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Microarray:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    MANN-WHITNEY:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Clone Assay:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Neutralization:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Cell Culture:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.

    Transformation Assay:

    Article Title: SARS-CoV-2 crossreactive B-cells outnumber seasonal coronavirus spike-specific clones at the end of the COVID-19 pandemic.
    Article Snippet: Briefly, B-cells were isolated from cryopreserved PBMCs using EasySep Human CD19 Positive Selection Kit (STEMCELL Technologies) according to manufacturer’s instructions.



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    Image Search Results


    Inverted fluorescence microscopy images of the cell lines utilised for the cultivation of HCoVs-229E, OC43, and NL63. The red frames in figures ( A – C ) denote CPE, which refer to the observable morphological and degenerative alterations occurring 3–7 days post-infection by HCoVs (for instance, clumped cell aggregates or rounded host cells): ( A ) LLC-MK2 cell line infected with HCoV-NL63; ( B ) HCT-8 cell line infected with HCoV-OC43; ( C ) MRC-5 cell line infected with HCoV-229E. The uninfected cell lines are represented as follows: ( D ) LLC-MK2; ( E ) HCT-8; and ( F ) MRC-5.

    Journal: International Journal of Molecular Sciences

    Article Title: Features and In Vitro Assessment of Antiviral Activity of Organic Coatings Doped with Silver-Based Compounds Against Human Coronavirus

    doi: 10.3390/ijms262211068

    Figure Lengend Snippet: Inverted fluorescence microscopy images of the cell lines utilised for the cultivation of HCoVs-229E, OC43, and NL63. The red frames in figures ( A – C ) denote CPE, which refer to the observable morphological and degenerative alterations occurring 3–7 days post-infection by HCoVs (for instance, clumped cell aggregates or rounded host cells): ( A ) LLC-MK2 cell line infected with HCoV-NL63; ( B ) HCT-8 cell line infected with HCoV-OC43; ( C ) MRC-5 cell line infected with HCoV-229E. The uninfected cell lines are represented as follows: ( D ) LLC-MK2; ( E ) HCT-8; and ( F ) MRC-5.

    Article Snippet: For virus stock multiplication, reference strains of HCoV, specifically 229E (ATCC-VR-740) (LGC Standards, Teddington, UK), OC43 (ATCC-VR-1558) (LGC Standards), and NL63 (Małopolskie Centrum Biotechnologii, Kraków, Poland) were cultivated on suitable adherent host cells: HCoV-229E/MRC-5 (Cytion), HCoV-OC43/HCT-8 (Cytion), and NL63/LLC-MK2 (Cytion) by incubating at temperatures between 33 and 35 °C with 5% CO 2 for a duration of 1–2 h to facilitate virus adsorption into the cells.

    Techniques: Fluorescence, Microscopy, Infection